Effect of verapamil on the proliferation of normal gingival fibroblast.
- Author:
Qing-xian LUAN
1
;
Cai-fang CAO
Author Information
- Publication Type:Journal Article
- MeSH: Calcium Channel Blockers; pharmacology; Cell Proliferation; drug effects; Cells, Cultured; Dose-Response Relationship, Drug; Fibroblasts; drug effects; Gingiva; cytology; Humans; Verapamil; pharmacology
- From: Chinese Journal of Stomatology 2007;42(1):31-33
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effect of verapamil on the proliferation of normal gingival fibroblast (NGF) in vitro.
METHODSNGF was isolated and cultured. The 5th passage of NGF was incubated with 0, 0.1, 1, 10, 100 micromol/L verapamil respectively. Methyl thiazolyl tetrazolium (MTT) assay and flow cytometry were used to detect cell proliferation and cell cycles.
RESULTSIncubated with 100 micromol/L verapamil for 66 h, the A value of normal gingival fibroblast was significantly lower than those without verapamil groups (P < 0.01). Incubated with 100 micromol/L verapamil for 18 h, 69% of cells were at the G(0) - G(1) phase, 27% were at the S phase. For control group (without verapamil) 41% of cells were at G(0) - G(1) phase and 49% cells were at S phase. There was significant difference between the two groups (P < 0.001).
CONCLUSIONS100 micromol/L verapamil inhibited proliferation of normal gingival fibroblast by a cell-cycle arrest.
