Production of interleukin-11 and interleukin-6 in cultured human gingival fibroblasts with the stimulation of lipopolysaccharides.
- Author:
Lu HE
1
;
Toshiyuki NAGASAWA
;
Isao ISHIKAWA
Author Information
- Publication Type:Journal Article
- MeSH: Aggregatibacter actinomycetemcomitans; chemistry; Cells, Cultured; Dose-Response Relationship, Drug; Escherichia coli; chemistry; Fibroblasts; drug effects; metabolism; Gingiva; cytology; metabolism; Humans; Indomethacin; pharmacology; Interleukin-11; biosynthesis; Interleukin-6; biosynthesis; Lipopolysaccharides; pharmacology; Porphyromonas gingivalis; chemistry
- From: Chinese Journal of Stomatology 2007;42(1):34-36
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of Porphyromonas gingivalis (Pg), Actinobacillus actinomycetemcomitans (Aa), Escherichia coli (Ec) lipopolysaccharides (LPS) on the production of IL-11 and IL-6 from healthy human gingival fibroblasts (HGF), and the effects of endogenous prostaglandin on HGF IL-11 and IL-6 production stimulated with the above LPS.
METHODSHGF were stimulated with Pg-, Aa-, Ec-LPS of different concentrations (0.1, 1, 10 mg/L) for 24 h. And HGF were also stimulated with the combinations of 10 mg/L Pg-, Aa-, Ec-LPS and 10(-6) mol/L indomethacin respectively for 24 h. Levels of IL-11 and IL-6 in the supernatants were quantitated by ELISA.
RESULTSLPS from Aa, Ec, at the concentration of 10 mg/L and from Pg at the concentrations 1, 10 mg/L significantly augmented IL-11 production by HGF. IL-6 production was also significantly increased by stimulation with Aa-LPS at concentrations 1, 10 mg/L and with Ec-, Pg-LPS at concentrations 0.1, 1, 10 mg/L. In addition, IL-11 production was lower than IL-6 production by HGF stimulated with LPS. Indomethacin significantly inhibited IL-6 and IL-11 production in LPS-stimulated HGF.
CONCLUSIONSAa-, Pg-, Ec-LPS may significantly increase IL-11 and IL-6 level in the supernatants of HGF, and endogenous prostaglandin may upregulate IL-11 and IL-6 production in LPS-stimulated HGF.