Experimental study on dog's bone marrow stem cells transfected by pIRES2-EGFP-IGF-1 gene.
- Author:
Guo-qiang ZHU
1
;
Zhi-fen WU
;
Yuan-fei LI
;
De-hua HU
;
Qin-tao WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow Cells; cytology; metabolism; Cells, Cultured; Dogs; Genetic Vectors; Insulin-Like Growth Factor I; genetics; metabolism; Male; Mesenchymal Stromal Cells; cytology; metabolism; Transfection
- From: Chinese Journal of Stomatology 2006;41(12):739-742
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the bone marrow stem cells (MSC) model which could highly express the insulin-like growth factor 1 (IGF-1) transfected by dog's IGF-1 gene.
METHODSpIRES2-EGFP-IGF-1 was transfected into MSC by lipofectamine. Positive clones were selected with G418. The expression of IGF-1 protein in the MSC was determined by immunohistochemistry and Western blot analysis. The IGF-1 in the supernatant of the transfected MSC was detected by sandwich-in ELISA. The periodontal ligament cells (PDLC) were cultured in the supernatant of the transfected MSC. The changes of PDLC' proliferation were observed by MTT.
RESULTSIGF-1-transfected MSC could apparently express IGF-1. The IGF-1 protein in the supernatant of the transfected MSC was confirmed by sandwich-in ELISA. IGF-1 could promote the PDLC' proliferation.
CONCLUSIONSThe MSC transfected by dog's IGF-1 gene can highly express IGF-1, which may lay the foundation for further study on periodontal regeneration.