Study on the inhibition of vascular endothelial growth factor expression in Tca8113 cell by inducible nitric oxide synthase gene RNA interference.
- Author:
Shu-guang ZENG
1
;
Wei-liang CHEN
;
Lan YANG
;
Wei-jian AI
;
Jin-cai ZHANG
;
Ji-ren ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Squamous Cell; genetics; metabolism; Cell Line, Tumor; Down-Regulation; Gene Expression Regulation, Neoplastic; Humans; Nitric Oxide Synthase Type II; genetics; RNA Interference; Tongue Neoplasms; genetics; metabolism; Vascular Endothelial Growth Factor A; genetics; metabolism
- From: Chinese Journal of Stomatology 2007;42(2):74-77
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of inducible nitric oxide synthase (iNOS) on vascular endothelial growth factor (VEGF) expression.
METHODSThe vector containing short hairpin RNA of iNOS was transfected into Tca8113 cells using the RNA interference (RNAi) technique. The gene and protein expression of iNOS and VEGF was examined by RT-PCR and Western blot.
RESULTSThe expression of iNOS, VEGF gene in Tca8113 cells was significantly different between the experimental and control groups 24 h and 48 h after transfection (P < 0.05). The protein expression of iNOS was different between the two groups 36 h and 48 h after transfection, and of VEGF was also different between the two groups 48 h after transfection (P < 0.05).
CONCLUSIONSThe expression of VEGF could be down-regulated by silencing the iNOS gene in Tca8113 cells.
