Effects of remifentanil on intracellular Ca(2+) and its transients induced by electrical stimulation and caffeine in rat ventricular myocytes.
- Author:
Ye ZHANG
1
;
Michael G IRWIN
;
Rui LI
;
Zhi-Wu CHEN
;
Tak-Ming WONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Caffeine; pharmacology; Calcium; metabolism; Calcium Signaling; drug effects; Cells, Cultured; Electric Stimulation; Male; Myocytes, Cardiac; drug effects; metabolism; Piperidines; pharmacology; Rats; Rats, Sprague-Dawley
- From: Chinese Medical Journal 2009;122(12):1439-1443
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDPreconditioning with remifentanil confers cardioprotection. Since Ca(2+) overload is a precipitating factor of injury, we determined the effects of remefentanil on intracellular Ca(2+) ([Ca(2+)](i)) and its transients induced by electrical stimulation and caffeine, which reflects Ca(2+) handling by Ca(2+) handling proteins, in rat ventricular myocytes.
METHODSFreshly isolated adult male Sprague-Dawley rat myocytes were loaded with Fura-2/AM and [Ca](i) was determined by spectrofluorometry. Remifentanil at 0.1 - 1000 microg/L was administered. Ten minutes after administration, either 0.2 Hz electrical stimulation was applied or 10 mmol/L caffeine was added. The [Ca(2+)](i), and the amplitude, time resting and 50% decay (t(50)) of both transients induced by electrical stimulation (E [Ca(2+)](i)) and caffeine (C [Ca(2+)](i)) were determined.
RESULTSRemifentanil (0.1 - 1000.0 microg/L) decreased the [Ca(2+)](i) in a dose-dependent manner. It also decreased the amplitude of both transients dose-dependently. Furthermore, it increased the time to peak and t(50) of both transients dose-dependently.
CONCLUSIONRemifentanil reduced the [Ca(2+)](i) and suppressed the transients induced by electrical stimulation and caffeine in rat ventricular myocytes.
