In vitro study of human multiple myeloma cell transfected with soluble vascular endothelial growth factor receptor-1 gene.
- Author:
Dong ZHENG
1
;
Juan LI
;
Jun-Ru LIU
;
Zhen-Hai ZHOU
;
Jing-Li GU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cell Proliferation; Enzyme-Linked Immunosorbent Assay; Gene Expression Regulation, Neoplastic; Humans; Multiple Myeloma; genetics; metabolism; pathology; Plasmids; Recombinant Proteins; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Transfection; Vascular Endothelial Growth Factor A; metabolism; Vascular Endothelial Growth Factor Receptor-1; genetics; metabolism; physiology
- From:Chinese Journal of Cancer 2010;29(1):65-68
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND AND OBJECTIVEVascular endothelial growth factor (VEGF) is the most important angiogenic factor of multiple myeloma (MM). This study was to investigate the effect of transfection of human soluble vascular endothelial growth factor receptor-1 (sFlt-1) gene on the proliferation of human MM cell line RPMI8226.
METHODSThe recombinant plasmid pcDNA3-sFlt-1 was constructed and transfected into RPMI8226 cells. The expression of sFlt-1 was identified by reverse transcription-polymerase chain reaction (RT-PCR) and ELISA. The effects of sFlt-1 protein on the proliferation and VEGF expression of RPMI8226 cells were investigated by MTT assay and ELISA, respectively.
RESULTSThe recombinant plasmid pcDNA3-sFlt-1 was successfully transfected into RPMI8226 cells. sFlt-1 protein expression was identified by ELISA, which inhibited the proliferation of RPMI8226 cells and reduced VEGF concentration in the culture supernatant.
CONCLUSIONRPMI8226 cells can express sFlt-1 protein with high biological activity when transfected with the sFlt-1 gene, which inhibits the proliferation of RPMI8226 cells.
