Grape seed extract induces morphological changes of prostate cancer PC-3 cells.
- Author:
Xue-Jun SHANG
1
;
Hong-Lin YIN
;
Jing-Ping GE
;
Yi SUN
;
Wen-Hui TENG
;
Yu-Feng HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Line, Tumor; drug effects; ultrastructure; Humans; Male; Microscopy, Electron, Transmission; Phytotherapy; Plant Extracts; pharmacology; Prostatic Neoplasms; drug therapy; Vitis
- From: National Journal of Andrology 2008;14(12):1090-1093
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the morphological changes of prostate cancer PC-3 cells induced by grape seed extract (GSE).
METHODSPC-3 cells were incubated with different concentrations of GSE (100, 200 and 300 microg/ml) for 24, 48 and 72 hours, and then observed for morphological changes by invert microscopy, HE staining and transmission electron microscopy.
RESULTSThe incubated PC-3 cells appeared round, small, wrinkled and broken under the invert microscope and exhibited the classical morphological characteristics of cell death under the electron microscope, including cell atrophy, increased vacuoles, crumpled nuclear membrane, and chromosome aggregation.
CONCLUSIONGSE can cause morphological changes and induce necrosis and apoptosis of PC-3 cells.
