Expression of Smad4 in prostate cancer LNCaP and ARCaP cell lines with different metastatic potentials and its significance.
- Author:
Kai-Jie WU
1
;
Dong ZHANG
;
Guang-Feng ZHU
;
He-Peng CHENG
;
Jin ZENG
;
Lin-Lin ZHANG
;
Xin-Yang WANG
;
Da-Lin HE
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Humans; Male; Neoplasm Metastasis; Prostatic Neoplasms; metabolism; pathology; RNA, Messenger; genetics; Smad4 Protein; biosynthesis; Transforming Growth Factor beta; metabolism
- From: National Journal of Andrology 2009;15(1):41-44
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the expression of Smad4, the core of TGF-beta/Smads signal transduction pathway in different prostate cancer cell lines, and explore their molecular mechanism of bone metastatic potential.
METHODSThe Millicell polycarbonate filter coated with matrigel was used to confirm the invasive potency of LNCaP and ARCaP cell lines (IF11 and IA8). The expressions of the Smad4 protein and mRNA in these prostate cancer cells with different metastatic potentials were detected by Western blotting and RT-PCR, respectively.
RESULTSARCaP cell lines (IF11 and IA8) exhibited a stronger potency of invasion than LNCaP (P < 0.01). The Smad4 protein and mRNA highly expressed in the LNCaP cell line that was well-known with a low metastatic potential, but not in the ARCaP (IF11 or IA8) cells with high metastatic potentials (P < 0.01).
CONCLUSIONSmad4 expresses differently in LNCaP and ARCaP cell lines with different metastatic potentials and, as a tumor suppressive gene, its deficient expression may play an important role in the invasion and metastasis of advanced prostate cancer.
