Identification of Wnt/beta-catenin signal pathway in human prostate cancer cell lines and its significance.
- Author:
Yong-Guang JIANG
1
;
Jia-Hui ZHAO
;
Yong LUO
;
Da-Lin HE
;
Xin-Yang WANG
;
Xin-Hao CUI
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Humans; Male; Prostatic Neoplasms; metabolism; Signal Transduction; Wnt Proteins; metabolism; beta Catenin; metabolism
- From: National Journal of Andrology 2009;15(4):310-313
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the Wnt/beta-catenin signal pathway in different human prostate cancer cell lines and explore its role in epithelial-mesenchymal transition (EMT) in human prostate cancer.
METHODSWe detected the expressions of beta-catenin, t-GSK3beta and p-GSK3beta in several prostate cancer cell lines (LNCaP, C4, C4-2, C4-2B, IF11, IA8, PC-3 and DU145) with different characteristics of epithelial-mesenchymal transition (EMT) by Western blotting.
RESULTSThere were remarkable differences in the expressions of beta-catenin and p-GSK3beta among the cell lines, with beta-catenin and p-GSK3beta highly expressed in LNCaP, C4, C4-2, C4-2B, IF11 and IA8, lowly expressed in PC-3 and DU145, but no difference was observed in the expressions of t-GSK3beta in all the cell lines.
CONCLUSIONThere are differences in the state of the Wnt/beta-catenin signal pathway among the cell lines with different characters of EMT.
