Construction of plasmid expression vector for specific peptide of the rubella virus E1 gene.
- Author:
Jing CAO
1
;
Yu-Feng HUANG
;
Jian GAO
;
Hao-Yang WANG
;
Jin-Chun LU
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; Cloning, Molecular; Gene Expression; Genetic Vectors; Molecular Sequence Data; Plasmids; RNA, Viral; Reverse Transcriptase Polymerase Chain Reaction; Rubella virus; genetics; immunology; Viral Envelope Proteins; genetics; immunology
- From: National Journal of Andrology 2009;15(4):318-321
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct a recombinant plasmid vector of the RV specific fragment for expressing the specific fragment of RV E1 protein.
METHODSRNA of the RV attenuated live vaccine Wistar RA27/3 strain was extracted and reversely transcribed. The specific fragment of the E1 gene was amplified and the PCR products cloned in the vector pGEX-2T after purification. Positive clones were selected and identified by two-enzyme digestion and sequence analysis.
RESULTSA 330 bp target fragment was successfully cloned, and the sequence of the recombinant plasmid was consistent with the original sequence.
CONCLUSIONSuccessful cloning of the RV El specific fragment and the construction of the recombinant plasmid have laid a foundation for further expressing the recombinant protein.
