Correlation between ERp57 and pregnancy of intrauterine insemination.
- Author:
Bin ZHANG
1
;
Zeng-Hui BIAN
;
Jian-Tang SU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Female; Humans; Insemination, Artificial; methods; Male; Pregnancy; Pregnancy Rate; Protein Disulfide-Isomerases; metabolism; Spermatozoa; metabolism
- From: National Journal of Andrology 2009;15(4):354-356
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the association between the pregnancy rate of intrauterine insemination (IUI) and the ERp57 expression level in donor sperm.
METHODSForty-two sperm samples were divided into 3 groups according to the pregnancy rate: Group A (n = 16, pregnancy rate = 0), B (n = 13, pregnancy rate 10%-20%) and C (n = 13, pregnancy rate > or = 20%). The optical density (OD) was normalized to the beta-tubulin band for the evaluation of the ERp57 level.
RESULTSOD (ERp57/Tubulin) was 0.95 +/- 0.24 in Group A, 1.33 +/- 0.43 in Group B and 1.33 +/- 0.39 in Group C. The ERp57 expression level was significantly lower in Group A than in B and C (P < 0.05), with no significant differences between the latter two groups.
CONCLUSIONThe ERp57 expression level in donor sperm could be used as an index to predict the pregnancy rate of IUI and to avoid IUI failure by removing low-level donor sperm.
