Analysis of microbial diversity of nitrifying bacteria by terminal restriction fragment length polymorphism.
- Author:
Weitie LIN
1
;
Yanan ZHU
Author Information
1. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China. wtlin@21cn.com
- Publication Type:Journal Article
- MeSH:
Animals;
Azoarcus;
genetics;
growth & development;
Bacteria;
classification;
genetics;
Biodiversity;
Bordetella;
genetics;
growth & development;
Brevibacillus;
genetics;
growth & development;
Nitrobacter;
classification;
genetics;
Pandalidae;
Polymorphism, Restriction Fragment Length;
Water Microbiology
- From:
Chinese Journal of Biotechnology
2010;26(4):483-488
- CountryChina
- Language:Chinese
-
Abstract:
We analyzed the microbial diversity and quantity of nitrifying bacteria in the enrichment reactor by Terminal Restriction Fragment Length Polymorphism (T_RFLP), a cultured-independent molecular technique. The result indicated that nitrobacteria enriched the best, and the diversity index decreased 62.80% compared with the initial data. Nitrobacteria were predominant in the reactor. Meanwhile, we studied the microbial diversity before and after adding Nitrobacteria into shrimp ponds, and analyzed several major bacterial species that existed stably in the pond. According to the analysis by T_RFLP program, species including Brevibacillus brevis, Microbacterium lactium, Azoarcus indigens and Bordetella holmesii were the dominant bacteria in the ponds.
