Preparation and properties of SiO2 tubes immobilized antibody for HCAg detection.
- Author:
Li XIE
1
;
Yueping GUAN
;
Ying GE
;
Hongbo SHI
Author Information
1. Beijing You'an Hospital, Capital Medical University, Beijing 100069, China. XL811CN@163.com
- Publication Type:Journal Article
- MeSH:
Antibodies, Immobilized;
immunology;
Antibodies, Monoclonal;
chemistry;
immunology;
Hepatitis C Antibodies;
chemistry;
immunology;
Hepatitis C Antigens;
analysis;
immunology;
Humans;
Silicon Dioxide;
chemistry
- From:
Chinese Journal of Biotechnology
2010;26(4):545-549
- CountryChina
- Language:Chinese
-
Abstract:
In order to optimize the fabrication of SiO2 tubes immobilized with antibody for hepatitis C virus antigen (HCAg) detection, we formed the activated amino on the surface of SiO2 tubes by using the activation of aminosilane. Then we immobilized the hepatitis C virus (HCV) monoclonal antibody on the surface of SiO2 tubes by using glutaraldehyde as a chemical cross-linker, followed by detecting HCAg. Sequence tests showed that when the SiO2 tubes were treated in 10% (V/V) aminosilane solution and 3% (V/V) glutaraldehyde solution for 3 hours and 2 hours, respectively, the HCV monoclonal antibody had high immobilization efficiency and low nonspecificity, and the HCAg was detected to 1 ng/mL. This experiment can provide principle and experimental data for establishment of HCAg magnetic immunoassay system.
