Genetic diversity in goat breeds based on microsatellite analysis.
- Author:
Limei XU
1
;
Chousheng LIU
;
Liping ZHANG
;
Zhigang WANG
;
Xu HAN
;
Xiaoxia LI
;
Shuang CHANG
Author Information
1. Gansu Agricultural University, Lanzhou 730070, China.
- Publication Type:Journal Article
- MeSH:
Alleles;
Animals;
Breeding;
DNA;
genetics;
Genetic Variation;
Goats;
classification;
genetics;
Heterozygote;
Microsatellite Repeats;
genetics;
Polymorphism, Genetic
- From:
Chinese Journal of Biotechnology
2010;26(5):588-594
- CountryChina
- Language:Chinese
-
Abstract:
Fluorescence PCR was applied to investigate the genetic diversities of 9 indigenous Chinese goat breeds and 1 exotic breed with 10 microsatellite DNA markers recommended by the Food and Agriculture Organization of the United Nations and the International Livestock Research Institute of Animal Genetics, which provide data for the preservation and utilization of indigenous goat breeds genetic resource. We found that the 7 breeds were high polymorphic while 3 breeds were moderate polymorphic. We also detected 119 alleles, and the effective allele number ranged from 1.4641 to 9.2911. The average heterozygosity of loci and breeds respectively varied from 0.2618 to 0.7672 and from 0.5196 to 0.7024. As well as SRCRSP23 site and Hexi cashmere goat had the highest average heterozygosity. Then we analyzed the phylogenetic trees (NJ and UPGMA), and found both of them were generally in accordance with their original breeding history and localities.
