Intergenus somatic hybridization between Alhagi pseudalhagi and Astragalus cicer by electroporation.
- Author:
Gaina ZHANG
1
;
Jingfen JIA
;
Xiangsheng KONG
;
Huawei XU
Author Information
1. College of Agriculture, Henan University of Science and Technology, Luoyang 471003, China. zgnluck1@163.com
- Publication Type:Journal Article
- MeSH:
Astragalus Plant;
genetics;
physiology;
Electroporation;
Fabaceae;
genetics;
physiology;
Hybridization, Genetic;
Transformation, Genetic
- From:
Chinese Journal of Biotechnology
2010;26(5):635-642
- CountryChina
- Language:Chinese
-
Abstract:
We obtained intergenus somatic hybrid between Alhagi pseudalhagi and Astragalus cicer by using electroporation. Agrobacterium rhizogenes A4-transformed A. pseudalhagi protoplasts were treated with iodoacetamide so that they were unable to sustain divisions. A. cicer protoplasts were isolated from a methionine-resistant mutant and did not survive in the medium without plant growth regulator. The intergenus somatic hybrid cells were selected based on physiological complementation between the two parents. We optimized some parameters of electroporation, such as direct current impulse, alternating current impulse and the impulse number. We identified ten hybrid clones by morphological observation, checking the chromosome numbers, isoenzyme analysis and random amplified polymorphic DNA analysis, and obtained regenerated plantlets from three hybrid clones.
