Cloning of keratinocyte growth factor 2 gene (KGF2) and its transformation to Brassica napus L.
- Author:
Guoqing PAN
1
;
Shuang ZHANG
;
Xiuming LIU
;
Ying LI
;
Yaofang ZHANG
;
Hongzhi LI
;
Haiyan LI
;
Xiaokun LI
Author Information
1. Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
- Publication Type:Journal Article
- MeSH:
Brassica napus;
genetics;
metabolism;
Cloning, Molecular;
DNA, Complementary;
genetics;
Fibroblast Growth Factor 7;
biosynthesis;
genetics;
Genetic Vectors;
genetics;
Humans;
Plants, Genetically Modified;
genetics;
Rhizobium;
genetics;
Transformation, Genetic
- From:
Chinese Journal of Biotechnology
2010;26(6):767-771
- CountryChina
- Language:Chinese
-
Abstract:
Recently, more research about the plant bioreactor expressing genes encoding human proteins was reported. In the present study, the cDNA of the human gene keratinocyte growth factor 2 (KGF2) was replaced with plant preferred codons by PCR, and the modified full-length cDNA was cloned into the plant expression vector pCAMBIA-YO containing the oil-body promoter. The fusion construct pCAMBIA-YO-KGF2 was transformed into Brassica napus by Agrobacterium tumefacien-mediated cotyledon transformation method. The transgenic seedlings were identified by PCR, Southern and western blot analysis all showed that KGF2 gene was successfully expressed in in transgenic Brassica napus.
