Preparation and verification of antibodies for five rice receptor-like kinases.
- Author:
Bo WANG
1
;
Liyun LI
;
Yinghao CAO
;
Ziguang LIU
;
Guozhen LIU
Author Information
1. College of Life Sciences, Agricultural University of Hebei, Baoding 071001, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Monoclonal;
biosynthesis;
immunology;
Cloning, Molecular;
Epitopes;
immunology;
Immunization;
Oryza;
enzymology;
genetics;
Plant Proteins;
genetics;
immunology;
Plantibodies;
immunology;
metabolism;
Protein Kinases;
genetics;
immunology;
Rabbits
- From:
Chinese Journal of Biotechnology
2010;26(6):796-801
- CountryChina
- Language:Chinese
-
Abstract:
Receptor-like kinase involves self-incompatibility, male sterility, stress responses, and disease resistance. To better understand the physiological function and biological characteristics of rice receptor-like kinase, we cloned five predicted epitope fragments of rice receptor-like kinase. The purified fusion protein was used as antigen to immunize rabbit to get specific polyclonal antibodies. Western blotting analysis shows that the five receptor-like kinases were expressed in rice leaves.
