Detection of bovine, goat, pig and chicken derived ingredients in animal products with universal PCR-microarray method.
- Author:
Fengyun SHI
1
;
Jiankun MIAO
;
Liping ZHANG
;
Hong TAO
;
Jianqiang LÜ
;
Zhouxi RUAN
;
Hui ZONG
Author Information
1. Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
- Publication Type:Journal Article
- MeSH:
Animal Feed;
analysis;
Animals;
Cattle;
Chickens;
DNA, Mitochondrial;
genetics;
Food Contamination;
analysis;
Goats;
Meat;
analysis;
Microarray Analysis;
methods;
Mitochondria, Muscle;
enzymology;
genetics;
Polymerase Chain Reaction;
methods;
RNA, Ribosomal, 16S;
genetics;
Sensitivity and Specificity;
Swine
- From:
Chinese Journal of Biotechnology
2010;26(6):823-829
- CountryChina
- Language:Chinese
-
Abstract:
We analyzed the sequence of vertebrate molecular marker genes, then we selected the mitochondrial DNA (mtDNA) 16S rRNA gene as marker gene. In order to detect four kinds of animal-derived ingredients, which including bovine, goat, pig and chicken. We utilized a pair of universal primers, designed four sets of species-specific microarray probes and two pairs of quality control probes. We optimized the PCR amplifications and hybridization conditions, therefore these four kinds of animal-derived ingredients could be rapid and accurate detected by this approach. The detection limits were all reaches 1 pg. We established the detection platform of these four kinds of animal-derived ingredients. This universal PCR-microarray assay provides a new method for the identification of animal-derived ingredients in the import-export field.
