Progress on biodiesel production with enzymatic catalysis in China.
- Author:
Tianwei TAN
1
;
Jike LU
;
Kaili NIE
;
Haixia ZHANG
;
Li DENG
;
Fang WANG
Author Information
1. Belting Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. twtan@mail.buct.edu.cn
- Publication Type:Journal Article
- MeSH:
Biofuels;
Bioreactors;
Biotechnology;
economics;
methods;
Candida;
enzymology;
Catalysis;
China;
Enzymes, Immobilized;
metabolism;
Esterification;
Fermentation;
Lipase;
metabolism;
Plant Oils;
chemistry
- From:
Chinese Journal of Biotechnology
2010;26(7):903-906
- CountryChina
- Language:Chinese
-
Abstract:
This paper reports the progress of biodiesel production with enzymatic catalysis in Beijing University of Chemical Technology, one of the leaders in biodiesel R & D in China, which includes screening of high-yield lipase production strains, optimization and scale-up of the lipase fermentation process, lipase immobilization, bioreactor development and scale-up, biodiesel separation and purification and the by-product glycerol utilization. Firstly, lipase fermentation was carried out at industrial scale with the 5 m3 stirred tank bioreactor, and the enzyme activity as high as 8 000 IU/mL was achieved by the species Candida sp. 99-125. Then, the lipase was purified and immobilized on textile membranes. Furthermore, biodiesel production was performed in the 5 m3 stirred tank bioreactor with an enzyme dosage as low as 0.42%, and biodiesel that met the German biodiesel standard was produced. And in the meantime, the byproduct glycerol was used for the production of 1,3-propanediol to partly offset the production cost of biodiesel, and 76.1 g/L 1,3-propanediol was obtained in 30 L fermentor with the species Klebsiella pneumoniae.
