Microbial lipid production by Rhodosporidium toruloides in a two-stage culture mode.
- Author:
Jintao LIN
1
;
Hongwei SHEN
;
Zehui ZHANG
;
Cuimin HU
;
Guojie JIN
;
Haidong TAN
;
Zongbao K ZHAO
Author Information
1. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
- Publication Type:Journal Article
- MeSH:
Basidiomycota;
growth & development;
metabolism;
Biofuels;
Cell Culture Techniques;
methods;
Fermentation;
Industrial Microbiology;
methods;
Lipids;
biosynthesis
- From:
Chinese Journal of Biotechnology
2010;26(7):997-1002
- CountryChina
- Language:Chinese
-
Abstract:
To shorten the cultivation time and reduce the consumption of raw materials for microbial lipid production, oleaginous yeast Rhodosporidium toruloides AS 2.1389 was cultivated using a two-stage culture mode, in which the cell propagation and lipid accumulation were separated. The yeast cells recovered from the propagation culture were re-suspended in glucose solution for lipid accumulation, through which lipid content over 55% of the dry cell weight was achieved, the longer the propagation stage was, the higher the lipid content. Analysis of the lipid indicated that the long-chain fatty acids with 16 and 18 carbon atoms were major components, suggesting that the lipid can be an alternative feedstock for biodiesel production.
