Screening of proteins interacting with avian influenza virus nucleoprotein by yeast two-hybrid system in human brain cDNA library.
- Author:
Yin WENSI
1
;
Hu YONG
;
Jin MEILIN
Author Information
1. State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
- Publication Type:Journal Article
- MeSH:
Brain;
Gene Library;
Humans;
Influenzavirus A;
chemistry;
genetics;
Nucleoproteins;
metabolism;
Protein Binding;
Protein Interaction Domains and Motifs;
Protein Interaction Mapping;
Two-Hybrid System Techniques
- From:
Chinese Journal of Biotechnology
2010;26(8):1037-1041
- CountryChina
- Language:Chinese
-
Abstract:
Avian influenza virus Nucleoprotein (NP) is important in viral transcription, replication and determining host specificity of influenza virus. Yeast two-hybrid technique was applied to screen for proteins interacting with virus nucleoprotein, so as to further elucidate the interaction between virus nucleoprotein and cellular proteins, as well as the interaction between virus and host. To explore new proteins interacted with NP protein, a human brain cDNA library was screened using yeast two-hybrid system with NP as the bait. DNA inserts of the positive AD/library plasmids were sequenced. By the BLAST analysis against the GenBank databases seven positive clones resulted in seven genes. Our results could help for the further study on the molecular mechanism of virus replication, transcription and protein-protein interaction. Further investigations were needed to characterize these interactions.
