Effect of pH on growth and lipid content of Chlorella vulgaris cultured in biogas slurry.
- Author:
Cui WANG
1
;
Huan LI
;
Qinqi WANG
;
Ping WEI
Author Information
1. College of Biotechnology and Pharmaceutical Engneering, Nanjing University of Technology, Nanjing 210009, China.
- Publication Type:Journal Article
- MeSH:
Biofuels;
Chlorella vulgaris;
growth & development;
metabolism;
Culture Media;
Culture Techniques;
methods;
Fermentation;
Hydrogen-Ion Concentration;
Lipids;
analysis;
biosynthesis
- From:
Chinese Journal of Biotechnology
2010;26(8):1074-1079
- CountryChina
- Language:Chinese
-
Abstract:
Using 50% biogas slurry as basic medium, we investigated the effect of pH on the growth and lipid accumulation of Chlorella vulgaris. Setting two-group experiments, one was only control the initial medium pH, the initial pH was set at 6.0, 6.5, 7.0, 7.5, 8.0, and 8.5, respectively. One was control the medium pH constant, set constant pH at 6.0, 6.5, 7.0, 7.5, 8.0, and 8.5, respectively. Using HCl and NaOH regulated the pH. Results showed that algae Chlorella vulgaris grows better at pH 6.5 and 7.0, accumulate the lipid at pH 7.0-8.5, so the optimal pH for the growth and the lipid accumulation of Chlorella vulgaris was 7.0. The average removal rate of nitrate from biogas slurry was 95%, phosphate was 97%.