Serum-free medium for suspension culture of recombinant Chinese hamster ovary (11G-S) cells.
- Author:
Xingmao LIU
1
;
Hong LIU
;
Lingling YE
;
Shichong LI
;
Benchuan WU
;
Haitao WANG
;
Jing XIE
;
Zhaolie CHEN
Author Information
1. Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China.
- Publication Type:Journal Article
- MeSH:
Animals;
CHO Cells;
Cell Culture Techniques;
methods;
Cricetinae;
Cricetulus;
Culture Media, Serum-Free;
Genetic Engineering;
Insulin;
pharmacology;
Recombinant Proteins;
biosynthesis;
genetics;
Transferrin;
pharmacology;
Urokinase-Type Plasminogen Activator;
biosynthesis;
genetics
- From:
Chinese Journal of Biotechnology
2010;26(8):1116-1122
- CountryChina
- Language:Chinese
-
Abstract:
With suspension adapted recombinant Chinese hamster ovary (CHO) cell lines 11G-S expressing human pro-urokinase (pro-UK) as the object of study, a serum-free medium for the cultivation of recombinant CHO cells in suspension was formulated by using Plackett-Burman design and response surface methodology. The two-level Plackett-Burman design was used to evaluate the effect of 10 medium supplements on the growth of the 11G-S cells in suspension culture. Among the 10 medium supplements, insulin, transferrin, and putrescine were identified as the most significant factors (P < 0.05). The response surface methodology with three factors and three levels was used to determine the optimal levels of these factors. And a serum-free medium, SFM-CHO-S for recombinant CHO cells suspension culture was formulated. The maximum cell density of 11G-S cells in SFM-CHO-S in suspension batch culture reached 4.12 x 10(6) cells/mL with a maximum pro-UK activity at 5614 IU/mL, which was superior to the commercial serum-free medium for recombinant CHO cells.
