Characterization and culture of isolated primary dairy goat mammary gland epithelial cells.
- Author:
Zhen WANG
1
;
Jun LUO
;
Wei WANG
;
Wangsheng ZHAO
;
Xianzi LIN
Author Information
1. College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling 712100, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Separation;
methods;
Cells, Cultured;
Epithelial Cells;
cytology;
Female;
Goats;
Mammary Glands, Animal;
cytology;
Primary Cell Culture;
methods
- From:
Chinese Journal of Biotechnology
2010;26(8):1123-1127
- CountryChina
- Language:Chinese
-
Abstract:
Based on the in vitro culturing system developed for epithelial cells in mammary gland of Xinong Saanen dairy goats using tissue explant culture, high density cultivation, and continuous passaging, the cultured epithelial cells were evaluated by growth curve fitting, karyotype analysis, immunofluorescence staining (keratin, epithelial membrane antigen (EMA), vimentin, beta-casein), oil red staining and RT-PCR of beta-casein gene. The results showed that the growth of epithelial cells with the model number of chromosome of 60 demonstrated a typical 'S' shape curve, the positive gene expression of keratin, EMA, vimentin and beta-casein was detected, the cytoplasmic lipid droplets were observed following the oil red staining, the cultured cells expressed the mRNA of beta-casein. In conclusion, the current in vitro culturing system can obtain the normal mammary gland epithelial cells with the function of secretion.
