Cartilage engineering utilizing a porous bioceramic (beta-TCP)-cell complex.
- Author:
Changyong WANG
1
;
Bin BO
;
Qiang ZHAO
;
Ximin GUO
;
Cuimi DUAN
;
Yonghong WANG
;
Ming FAN
;
Jianxi LU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcium Phosphates; pharmacology; Cartilage; growth & development; transplantation; DNA; analysis; Female; Glycosaminoglycans; analysis; Immunohistochemistry; Mice; Mice, Nude; Tissue Engineering
- From: Chinese Journal of Plastic Surgery 2002;18(1):9-11
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the feasibility of growing tissue-engineered cartilage using chondrocytes seeded onto a biodegradable porous bioceramic, the beta-tricalcium phosphate (beta-TCP).
METHODSA porous bioceramic template of beta-TCP was created in the shape of a disc. Chondrocytes isolated from rabbit articular cartilage were seeded on the beta-TCP template and then kept in rotatory cell culture system (RCCS) for 1 week prior to subcutaneous transplantation into athymic mice. The three-dimensional structure was well-maintained 16 weeks after implantation. After 4, 8, 16 weeks, the specimens were harvested and examined macroscopically, histologically and immunohistochemically.
RESULTSGross morphological and histological analysis of the specimens from the chondrocyte-beta-TCP complex demonstrated new cartilage construction. The overall configuration of the experimental specimens closely resembled the structure of beta-TCP template.
CONCLUSIONThese findings suggest that porous bioceramic (beta-TCP) is a good "matrix" for chondrocyte, and can be used for cartilage engineering.
