Effects of microfilaments on the expression of collagenases and tissue inhibitor of metalloproteinases-1 in fibroblasts.
- Author:
Haifeng GU
1
;
Qinglian HE
;
Zihao LIN
;
Qi LIU
;
Xingmei ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Actin Cytoskeleton; physiology; Cells, Cultured; Collagenases; genetics; Fibroblasts; metabolism; Humans; RNA, Messenger; analysis; Tissue Inhibitor of Metalloproteinase-1; genetics; Wound Healing; physiology
- From: Chinese Journal of Plastic Surgery 2002;18(3):163-164
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the roles of microfilaments in the expression of collagenase and TIMP-1 in normal and hypertrophic scar.
METHODSCell culture and Northern blot hybridization methods were used in this study.
RESULTSAfter the disruption of microfilaments with cytochalasin B, mRNA contents of collagenase and TIMP-1 both increased significantly in normal and hypertrophic scar fibroblasts.
CONCLUSIONThe result suggest that the microfilament cytoskeleton may regulate the synthesis and degradation of ECM, which may be a mechanism of gene regulation during wound healing.
