Effects of conditioned medium derived from different keratinocytes on proliferation and collagen synthesis of hypertrophic scar fibroblasts.
- Author:
Shuzhong GUO
1
;
Linxi ZHANG
;
Zhen WANG
;
Jianbo LIU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Division; Cells, Cultured; Cicatrix, Hypertrophic; metabolism; pathology; Collagen; biosynthesis; Culture Media, Conditioned; Fibroblasts; physiology; Humans; Keratinocytes; physiology
- From: Chinese Journal of Plastic Surgery 2002;18(2):83-85
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of supernatants of normal skin keratinocytes(NK) and scar keratinocytes(SK) on proliferation and collagen synthesis of hypertrophic scar fibroblasts(HSFB).
METHODSThe supernatant, collected from cultured NK and SK, was added to the cultivated HSFB. The MTT-method, 3H-proline incorporation and radioimmunoassay were employed to measure the cell proliferation, collagen synthesis and secretion.
RESULTSNK supernatant could inhibit HSFB proliferation and increase the collagen synthesis, but inhibit collagen secretion, as compared with the control group. On the contrary, SK supernatant could increase collagen synthesis and secretion, which had little effects on HSFB proliferation.
CONCLUSIONKeratinocytes derived from normal skin and hypertrophic scar show different effects on hypertrophic scar fibroblasts.
