The experimental study on antisense TGF-beta 1 in inhibiting scar formation in III degree burn wound.
- Author:
Xiaoping LUO
1
;
Hanfang ZHAO
;
Youzhang ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Burns; drug therapy; physiopathology; Cicatrix; prevention & control; Male; Oligonucleotides, Antisense; therapeutic use; Rats; Rats, Sprague-Dawley; Transforming Growth Factor beta; antagonists & inhibitors; Transforming Growth Factor beta1; Wound Healing; physiology
- From: Chinese Journal of Plastic Surgery 2002;18(2):89-91
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of antisense TGF-beta 1 in inhibiting scar formation during wound healing.
METHODSSD rats were divided into three groups after skin burn: group one was treated with antisense TGF-beta 1 oligonucleotide; group two was treated with antisense TGF-beta 1 recombinant plasmid and the control group. In different time, RT-PCR and immunohistochemistry were used to verify the expression of TGB beta 1 mRNA and protein. Type I Collagen mRNA expression was determined by hybridization in situ. Inflammatory reaction and collagen distribution were observed pathologically.
RESULTSIn the groups received antisense ODN and recombinant plasmid, the expression of TGF-beta 1 mRNA and protein reduced during 14 days after burn. In the control group, type I collagen mRNA began to express at the second week and reached a peak at the fourth week, while the antisense groups kept low expression. The antisense group also showed mild inflammatory reaction and less synthesis of collagen.
CONCLUSIONAntisense TGF-beta 1 could prevent the scar formation during wound healing.
