The experimental study of culture in vitro of fibroblasts seeded onto human amnion extracellular matrix (HA-ECM).
- Author:
Qingyi HE
1
;
Bingli CHEN
;
Zhibiao WANG
;
Qihong LI
Author Information
- Publication Type:Journal Article
- MeSH: Amnion; cytology; Animals; Cell Culture Techniques; methods; Cells, Cultured; Extracellular Matrix; Female; Fibroblasts; cytology; Hematoxylin; Humans; Microscopy, Electron, Scanning; methods; Rabbits
- From: Chinese Journal of Plastic Surgery 2002;18(4):229-231
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe aim of this experiment is to find proper cell carrier for skin tissue engineering.
METHODSVarious concentration of fibroblasts were seeded onto HA-ECM and cultured in vitro. The performance of cells' growth, array, adhesion and collagen secretion on HA-ECM was observed with light microscope and transmission electron microscope.
RESULTSThe fusiform fibroblasts oriented radiantly or longitudinally and closely packed onto the HA-ECM, they attached firmly and proliferated to confluence on the stromal surface of HA-ECM.
CONCLUSIONThe optimal cell concentration is 3.5 x 10(6)/ml, HA-ECM is ideal carrier for fibroblasts because of its excellent scaffold and diffusion characteristics. Futhermore, it has the bioactive molecules such as fibronectin and laminin which play an important role in fibroblasts attachment and proliferation on HA-ECM.
