An experimental study on c-myc gene mRNA expression of endothelial cell induced by bFGF.
- Author:
Zhigang ZHOU
1
;
Qinglian HE
;
Zihao LIN
;
Songlin YANG
;
Xiaohai ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Division; drug effects; Cells, Cultured; Dose-Response Relationship, Drug; Endothelium, Vascular; cytology; drug effects; metabolism; Fibroblast Growth Factor 2; pharmacology; Gene Expression Regulation; drug effects; Genes, myc; genetics; RNA, Messenger; genetics; metabolism; Rats; Rats, Sprague-Dawley; Time Factors
- From: Chinese Journal of Plastic Surgery 2002;18(5):299-301
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the molecular biological mechanism of endothelial cell proliferation induced by bFGF.
METHODSCultured rat myocardial microvascular endothelial cells were stimulated with bFGF of various concentrations. By northern blot analysis, the levels of c-myc mRNA expression were detected.
RESULTSThe expression of c-myc mRNA in the bFGF-treated groups increased (P < 0.05) with a dose- and stimulating time-dependent manner. The c-myc mRNA expression reached to a peak level at 2 hours.
CONCLUSIONSC-myc expression may be an important component in controlling the transit of cells through the cell cycle.
