Influence of endothelial cells on the proliferation of scar-derived fibroblast in hypertropic scar tissue.
- Author:
Linxi ZHANG
1
;
Shuzhong GUO
;
Wensen XIA
;
Li YANG
;
Zhen WANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Cycle; drug effects; Cell Division; drug effects; Cell Line; Cells, Cultured; Cicatrix, Hypertrophic; pathology; Culture Media, Conditioned; chemistry; pharmacology; Dose-Response Relationship, Drug; Endothelium, Vascular; chemistry; cytology; Fibroblasts; drug effects; pathology; Humans
- From: Chinese Journal of Plastic Surgery 2002;18(6):338-340
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the influence of the endothelial cells on the scar-derived fibroblasts in the hypertropic scar tissue.
METHODSThe endothelial cell from the human umbilical vein was cultured and its supernatant was then collected. Thereafter, it was mixed with the pre-cultured scar-derived fibroblasts from the hypertropic scar tissue. The fibroblasts were analyzed with a flow cytometer in a MTT-assay method to evaluate the cell proliferation and its division cycle.
RESULTSThe endothelial cells were significantly increasing the scar-derived fibroblast proliferation (P < 0.05), compared with the control. The proportion of the fibroblasts in the S-stage of cell cycle was also quite high.
CONCLUSIONThe endothelial cells may secrete some active products, which may enhance the proliferation of the scar-derived fibroblasts and stimulate the fibroblasts into the S-stage of cell generation cycle.
