Curcumin attenuates contrast-induced nephropathy by upregulating heme oxygenase-1 expression in rat
10.3760/cma.j.issn.0253-3758.2013.02.008
- VernacularTitle:姜黄素预诱导血红素氧合酶1表达防治对比剂肾病的实验研究
- Author:
Bing-Jun DUAN
1
;
Le HUANG
;
Hong DING
;
Wei-Yi HUANG
Author Information
1. 内江市第一人民医院心内科
- Keywords:
Curcumin;
Heine oxygenase-1;
Contrast media;
Nephrosis
- From:
Chinese Journal of Cardiology
2013;41(2):116-120
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of curcumin (CMN) on contrast-induced nephropathy (CIN) in rats and explore the potential mechanisms focusing on heme oxygenase-1 (HO-1)expression.Methods Male SD rats (n =24) were randomly divided into four groups (n =6 each): control group (group A),diatrizoate group(DTZ,group B),DTZ + CMN group (group C),DTZ + CMN + zinc protoporphyrin Ⅸ group (group D).All rats were fed with normal chow for 1 week,right kidney was excised under anesthesia and rats were fed with normal chow for another 4 weeks.Mterwards,rats in group A was fed with normal chow,and rats in group B to D were fed with low-salt diet.All rats were injected furosemide 2 mg · kg-1 · d-1 for 7 days intramuscularly.At the beginning of the 7th day,rats in group C were injected intramuscularly with CMN 20 mg/kg,rats in group D were injected with CMN (20 mg/kg) + zinc protoporphyrin Ⅸ (7.5 mg/kg) while rats in group A and B were injected with equal volume of physiological saline.At the end of the 7th day,indometacin (10 mg/kg) was injected into tail vein of all rats.One hour later,60％ DTZ (8 ml/kg) was injected to rats in the group B,C and D while equal volume saline was injected to rats in group A through common carotid artery.After 48 hours,blood was drawn from the hearts of deeply anesthetized rats and kidney tissue was obtained for histology,HO-1,Bax,Bcl-2 expression and the apoptotic index measurements.Results The serum creatinine of group B,C and D [(83.67 ± 4.50)μmol/L,(63.67 ±4.76) μmol/L,(104.17 ±4.58) μmol/L] was significantly higher than that of group A [(41.50 ±5.58) μmol/L,all P ＜0.05],the serum creatinine was significantly higher in group B than in group C and lower than in group D (all P ＜ 0.05).HO-1 expression of group B,C and D was significantly higher than that of group A(all P ＜0.05),significantly higher in group C than in group B and D (all P ＜0.05).HO-1 activity of group A,B and C was significantly higher than that of group D (all P ＜ 0.05),HO-1 activity was significantly higher in group B than in group A and significantly lower in group B than in group C (all P ＜ 0.05).Bax,Bcl-2 expression and apoptosis index of group B,C and D were significantly higher than that of group A (all P ＜ 0.05),while Bcl-2/Bax of group B,C and D were significantly lower than that of group A(all P ＜ 0.05).Bcl-2 and Bcl-2/Bax were significantly higher while apoptosis index was significantly lower in group C than in group B (all P ＜ 0.05).Bax and apoptosis index were significantly higher and Bcl-2,Bcl-2/Bax were significantly lower in group D than in group B(all P ＜ 0.05).Conclusion CMN could protect against contrast-induced nephropathy through reducing renal cell apoptosis via upregulating HO-1 expression and activating HO-1 activity in rats.
