Poly (ADP-ribose) polymerase contributes myocardial ischemia-reperfusion of rats by regulating Akt signaling pathway
10.3760/cma.j.issn.0253-3758.2013.02.015
- VernacularTitle:在大鼠心肌缺血再灌注中多二磷酸腺苷-核糖聚合酶通过调节Akt信号通路造成心肌损伤
- Author:
Zhao-Feng SONG
1
Author Information
1. 271000,山东省泰安市中心医院心内科
- Keywords:
Myocardial reperfusion injury;
Poly (ADP-ribose) polymerases;
Protein kinases;
Signal transduction
- From:
Chinese Journal of Cardiology
2013;41(2):156-160
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of poly (ADP-ribose) polymerase(PARP) in heart ischemia and reperfusion (I/R) injury in rat and on Akt mediated signaling pathway.Method Rats were divided into sham,I/R,I/R + 3,4-dihydro-5-[4-(1-piperidinyl) butoxy]-1 (2H)-isoquinolinone (DPQ,10 mg/kg,i.p.),an inhibitor of PARP,I/R + DPQ + Akt inhibitor LY294002,10 mg/kg (n =12 each).Cardiac function,apoptosis of the cardiomyocytes were measured,myocardial expression of PARP,Akt,glycogen synthase kinase-3β (GSK-3β) and forkhead transcription factor FOXO3a were detected.Results (1) The expression of PARP were significantly upregulated in I/R group compared to sham group which was significantly attenuated in I/R + DPQ group(P ＜ 0.05 vs.I/R group).(2)PARP inhibition significantly reduced cardiomyocyte apoptosis from (34.0 ± 6.2) ％ to (23.0 ± 3.8) ％ (P ＜0.05).The LVDP,+ dp/dt and-dp/dt were significantly higher in I/R + DPQ group compared to I/R group (all P ＜ 0.05).(3) The expression of Akt,GSK-3β and FOXO3a were significantly upregulated in I/R + DPQ group compared to I/R group(P ＜0.05) which were significantly attenuated in I/R + DPQ + LY294002 group compared to I/R + DPQ group(all P ＜ 0.05).Conclusion PARP activation contributes to myocardial I/R injury in rats by modulating Akt mediated signaling pathway.
