Effects of perindopril on expression of kidney aquaporin-2 and urine aquaporin-2 excretion in chronic heart failure rats
10.3760/cma.j.issn.0253-3758.2013.04.004
- VernacularTitle:培哚普利对慢性心力衰竭大鼠肾脏水通道蛋白2的影响
- Author:
Shao OUYANG
1
;
Wei CHEN
;
Xi-Bin KUANG
Author Information
1. 南华大学附属第二医院心血管内科
- Keywords:
Heart failure,congestive;
Aquaporin-2;
Perindopril
- From:
Chinese Journal of Cardiology
2013;41(4):276-281
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the expression of kidney aquaporin-2 (AQP2) and urine AQP2 excretion in chronic heart failure (CHF) rats and investigate effects of perindopril on the expression and excretion of AQP2.Methods Sixty rats were randomized into three groups:control group,CHF group,CHF + Perindopril group.According to left ventricular myocardial infarction size,CHF group and perindopril group were further divided into heart failure subgroup (LVMI ≥ 20％)and cardiac functional compensation subgroup(LVMI ＜ 20％),respectively.Blood and urine samples were collected from the rats for measuring serum Na+,urine volume and urine osmolality.The concentration of plasma arginine vasopressin (p-AVP) was detected by radioimmunoassay (RIA).Immunohistochemistry,semi-quantitative real time-polymerase chain reaction (RT-PCR) and Western blot were performed for measurement of kidney inner medullary AQP2.The concentration of Urine AQP2 was measured by indirect enzyme-linked immunosorbent assay (indirect ELISA).Results Immunohistochemistry,RT-PCR,Western blot examinations revealed increased quantity of the inner kidney medullary AQP2 expression (0.2013 ± 0.0417),AQP2 mRNA (0.98 ± 0.33)and AQP2 protein expression(0.94 ± 0.21) in heart failure subgroup (n =13) compared to control group (n =20,0.1518 ±0.0214,0.58 ±0.51,0.51 ±0.46),which could be significantly by perindopril(n =13,0.0712 ±0.0218,0.76 ±0.45,0.82 ±0.49,all P ＜0.05 vs.heart failure subgroup).The concentration of plasma arginine AVP[(19.72 ±3.91) ng/ml] and Urine AQP2[(82.52 ± 11.77) ng/L] were significantly higher in heart failure subgroup than in control group [n =20,(51.67 ± 12.58) ng/L,(6.94 ± 3.10)ng/ml] (P ＜ 0.05),which were significantly reduced by perindopril [n =13,(15.65 ± 4.10) ng/L,(71.65 ±9.21) ng/ml].Conclusion Increased expression of the kidney inner medullary AQP2 and the excretion of urine AQP2 in chronic heart failure rats could be reduced by perindopril.
