Effects of fluoride on osteoclastic activity of rats in vitro.
- Author:
Kun HUA
1
;
Li-sha BU
;
Guang-sheng LI
Author Information
- Publication Type:Journal Article
- MeSH: Acid Phosphatase; metabolism; Animals; Animals, Newborn; Biomarkers; Cells, Cultured; Dose-Response Relationship, Drug; Fluorides; pharmacology; Isoenzymes; metabolism; Matrix Metalloproteinase 9; biosynthesis; genetics; Osteoclasts; cytology; drug effects; enzymology; RNA, Messenger; biosynthesis; genetics; Rats; Rats, Wistar; Tartrate-Resistant Acid Phosphatase
- From: Chinese Journal of Preventive Medicine 2003;37(4):256-258
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of fluoride on activities of tartrate-resistant acid phosphate (TRAP) and matrix metalloproteinase-9 (MMP-9) in rat osteoclasts cultured in vitro.
METHODSOsteoclast was isolated mechanically from long bones of neonatal rats and cultured in vitro. Histochemical stain was applied to detect the effects of fluoride on activities of TRAP and in-situ hybridization was used to study the expression of MMP-9 mRNA in rat osteoclasts in vitro.
RESULTSNumber of TRAP positive cells was 154.2, 160.0, 170.6, 179.0 and 180.0 per cm(2), respectively for the rats with varied doses of fluoride, in a dose-response pattern but without statistical significance. The expression of MMP-9 mRNA increased with elevating dose of fluoride, especially in the rats with 1.00, 2.00 and 4.00 mg/L of fluoride, to 94.50, 94.64 and 104.97, respectively, significantly different from those in control group.
CONCLUSIONSFluoride can enhance the MMP-9 mRNA expression in cultured osteoclasts of rats.
