Noise magnetic fields block co-suppression effect induced by power frequency magnetic field and phorbol ester.
- Author:
Xiang-wei GAO
1
;
Zheng-ping XU
;
Ya-nan HUO
;
Huai JIANG
;
Yi-ti FU
;
De-qiang LU
;
Qun-li ZENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Communication; drug effects; physiology; radiation effects; Cell Line; Electromagnetic Fields; adverse effects; Fluorescence Recovery After Photobleaching; Gap Junctions; drug effects; physiology; radiation effects; Mice; NIH 3T3 Cells; Noise; adverse effects; Tetradecanoylphorbol Acetate; pharmacology
- From: Chinese Journal of Preventive Medicine 2004;38(1):11-13
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo explore intervention with electromagnetic noise for co-suppression effect on gap-junctional intercellular communication (GJIC) induced or strengthened by low intensity magnetic field with carcinogen 12-O-tetradecanoylphorbol-13-acetate (TPA).
METHODSFibroblast cells from NIH 3T3 mice were exposed to extremely low intensity magnetic field (MF) 0.2 mT, 0.2 mT + TPA or/and electromagnetic noise with the same intensity of MF for 24 h, and GJIC was determined using fluorescence recovery analysis after photobleaching (FRAP) with a laser-scanning confocal microscope (Leica, Germany).
RESULTSGJIC function could be co-suppressed by MF of 0.2 mT with TPA, with fluorescence recovery of (23 +/- 11)%, lower than that in the control group [(46 +/- 19)%] and in the group with TPA only [(34 +/- 17) %] (P < 0.01), indicating 0.2 mT MF plus TPA could co-inhibit GJIC (P < 0.01). Superposition of 0.2 mT noise MF could get a fluorescence recovery of (35 +/- 19)% and significantly antagonize its co-suppression by TPA.
CONCLUSIONElectromagnetic noise of 0.2 mT could block the intensifying effect of power frequency magnetic field on TPA-induced GJIC inhibition.
