Study on biology and epidemiological characteristics of vibrio cholerae non-O1 strains isolated from environmental waters in Foshan city.
- Author:
Ai-zhen CHEN
1
;
Dai-song CAI
;
Su-yi ZHU
;
Zu-xing HUANG
;
Hua-le LIAO
;
Guo-you YAN
;
Wen ZHU
Author Information
- Publication Type:Journal Article
- MeSH: China; Enterotoxins; genetics; Environmental Monitoring; methods; Enzyme-Linked Immunosorbent Assay; Polymerase Chain Reaction; Seasons; Serotyping; Vibrio cholerae non-O1; classification; genetics; isolation & purification; Water; analysis; Water Microbiology
- From: Chinese Journal of Preventive Medicine 2004;38(1):47-49
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the distribution of serotype and the positive rate of toxins among vibrio cholerae non-O(1) isolated from environmental waters in Foshan city.
METHODSWater specimens were collected from river and cultured for vibrio cholerae non-O(1). The PCR method was used to detect cholerae enterotoxin (CT) gene; the ELISA method was used to detect heat-stable toxin (ST) and heat-labile toxin (LT).
RESULTS478 vibrio cholerae non-O(1) strains were isolated from 1 644 water specimens, with a positive rate of 29.07%. Serological assay showed that the main serotype of vibrio cholerae non-O(1) in Foshan city is VBO(7). Positive rate of CT, ST and LT were 1.91%, 13.14% and 12.17%, respectively.
CONCLUSIONSA few non-O(1) strains were found to have several virulent factors simultaneously, and the results suggest that vibrio cholerae non-O(1) in environmental waters is potentially pathogenic and may affect people's health. It is necessary to pay attention to the prevention of diarrhoea caused by vibrio cholerae.
