Detection for severe acute respiratory syndrome (SARS) coronavirus RNA in stool of SARS patients.
- Author:
Zhong-ping HE
1
;
Qing-ming DONG
;
Shu-jing SONG
;
Lin HE
;
Hui ZHUANG
Author Information
- Publication Type:Journal Article
- MeSH: China; Feces; virology; Humans; Polymerase Chain Reaction; RNA, Viral; genetics; metabolism; SARS Virus; genetics; isolation & purification; Severe Acute Respiratory Syndrome; virology
- From: Chinese Journal of Preventive Medicine 2004;38(2):90-91
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate excretion of severe acute respiratory syndrome coronavirus RNA (SARS-CoV) in stool of SARS patients.
METHODSSARS-CoV RNA was detected in stool specimens with fluorescent quantitative polymerase chain reactions (FQ-PCR) in 101 SARS patients on the 10 to 55 days after onset, 27 non-SARS patients and 400 individuals with health check-up.
RESULTSSARS-CoV RNA was positive in stool specimens by FQ-PCR in 58 of 101 SARS patients (57.4%), and all negative in 27 non-SARS patients and 400 healthy individuals. Positive rate of SARS-CoV RNA was 100% (8/8), 67.7% (21/31), 47.4% (27/57) and 40.0% (2/5) on the 10 - 19, 20 - 29, 30 - 39 and 40 - 55 days after onset of fever, respectively, with values of logarithm of SARS-CoV RNA load of 6.06 +/- 2.05, 4.51 +/- 1.23, 3.82 +/- 1.44 and 3.57 +/- 1.25, respectively.
CONCLUSIONPositive rate and load of SARS-CoV RNA in stool of SARS patients was the highest at their acute phase, and decreased with the extension of its course.
