The effects of conjugated linoleic acid on the ability of murine macrophage in killing tumor cells.
- Author:
Xuan-lin WANG
1
;
Bing-qing CHEN
;
Yan-mei YANG
;
Yu-mei ZHENG
;
Jia-ren LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blotting, Western; Cell Division; Cell Line, Tumor; Coculture Techniques; Dose-Response Relationship, Drug; Interleukin-6; genetics; Linoleic Acids, Conjugated; pharmacology; Macrophages; drug effects; physiology; Melanoma, Experimental; pathology; Mice; Mice, Inbred C57BL; Mitogen-Activated Protein Kinases; metabolism; Nitric Oxide Synthase; genetics; RNA, Messenger; drug effects; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Tumor Necrosis Factor-alpha; genetics
- From: Chinese Journal of Preventive Medicine 2004;38(2):107-110
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of c9,t11-conjugated linoleic acid on the killing ability of macrophage to B16-MB cells in C57 mice and explore its possible mechanism.
METHODSThe five levels of CLA was designed as 0, 25, 50, 75, 100 micro mol/L. After macrophage was treated with CLA for 24 h, the killing ability of macrophage on B16-MB cells was evaluated by MTT, The expression of C57 mice macrophage cytokine IL-6, TNF-alpha and iNOS mRNA was detected by RT-PCR. The expression of Erk protein was examined by Western Blot assay.
RESULTSThe inhibitory effect of macrophage on tumor cell depend on the treatment of the increased c9,t11-CLA level, at the same time, the expression of IL-6, TNF-alpha and iNOS mRNA increased, the expression of Erk decreased with the elevating dose of CLA.
CONCLUSIONSc9,t11-CLA could increase the killing ability of macrophage in mice to B16-MB cells, and it was associated with induction of IL-6, TNF-alpha and iNOS mRNA expression. We speculate that antitumor ability of CLA may be associated with taking part in body immune regulation action, and the effects of CLA on the killing ability of murine macrophage to B16-MB cells was not associated with the MAPKErk pathway.
