Effects of tea polyphenols and tea pigments on telomerase activity of HepG2 cells.
- Author:
Xu-dong JIA
1
;
Chi HAN
;
Jun-shi CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Biomarkers, Tumor; metabolism; Carcinoma, Hepatocellular; enzymology; pathology; Flavonoids; isolation & purification; pharmacology; Humans; Liver Neoplasms; enzymology; pathology; Phenols; isolation & purification; pharmacology; Pigments, Biological; isolation & purification; pharmacology; Polyphenols; Tea; chemistry; Telomerase; metabolism; Tumor Cells, Cultured
- From: Chinese Journal of Preventive Medicine 2004;38(3):159-161
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThis study is to investigate the effect of tea polyphenols and tea pigments on telomerase activity of human liver cancer cell line, HepG2 cells.
METHODSTRAP-PCR-ELISA was applied to investigate the telomerase activity.
RESULTSTelomerase was positive in tea polyphenols treated groups, tea pigments treated groups and blank control group. Telomerase activities (A(450 approximately 690) values) were 1.56 and 1.46 in 50 mg/L and 100 mg/L tea polyphenols-treated groups, 1.55 and 1.49 in 50 mg/L and 100 mg/L tea pigments-treated groups, respectively. The results showed that telomerase activity was significantly inhibited by tea polyphenols and tea pigments treatment as compared with the blank control group (A(450 approximately 690) = 2.11).
CONCLUSIONSTea polyphenols and tea pigments could significantly inhibit telomerase activity of HepG2 cells, and telomerase activity may be a useful biomarker for cancer chemoprevention.
