Study of genetic polymorphisms of 12 short tandem repeats on X chromosome in ethnic Han population from Hebei Province.
- Author:
Jie XU
1
;
Liang LEI
;
Ning XU
;
Guangping FU
;
Zhilu XU
;
Yuan WANG
;
Shujin LI
;
Bin CONG
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; Asian Continental Ancestry Group; ethnology; genetics; China; Chromosomes, Human, X; genetics; Female; Genetics, Population; Humans; Male; Microsatellite Repeats; Polymerase Chain Reaction; Polymorphism, Genetic
- From: Chinese Journal of Medical Genetics 2014;31(3):388-392
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate genetic polymorphisms of 12 X chromosome short tandem repeat (X-STR) loci in ethnic Hebei Han population using an Investigator Argus X-12 amplification kit.
METHODSDNA was extracted for 198 unrelated individuals (96 males and 102 females) and amplified with a fluorescence labeled multiplex PCR system. PCR products were separated and genotyped with capillary array electrophoresis.
RESULTSOnly DXS10103 and DXS10101 showed significant linkage disequilibrium at the 12 X-STR loci. One hundred and forty-eight alleles, including 22 off-ladder (OL) alleles, were observed at the 12 X-STR loci in the population. The heterozygosity and polymorphic information content (PIC) were 0.5074-0.9143 and 0.4377-0.9079, respectively. The power of discrimination (PD) was 0.5074-0.9143 in males and 0.6876-0.9863 in females. The mean exclusion chance was 0.4377-0.9079 in the trios cases and 0.2984-0.8373 in the duo cases, respectively.
CONCLUSIONThe Investigator Argus X12 amplification system is highly polymorphic in ethnic Han population from Hebei and is useful for personal identification and paternity testing.
