Confirmation of 17 rare HLA alleles and prediction of their haplotypes.
- Author:
Chuanfu ZHU
1
,
2
;
Yi ZHANG
;
Xiangmin NIE
;
Wenben QIAO
;
Yonghong SONG
;
Yan LIU
;
Yunlong ZHUANG
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; Asian Continental Ancestry Group; ethnology; genetics; Gene Frequency; HLA-A Antigens; genetics; HLA-B Antigens; genetics; HLA-DRB1 Chains; genetics; Haplotypes; Humans
- From: Chinese Journal of Medical Genetics 2014;31(4):518-521
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo confirm 17 rare HLA alleles detected during routine HLA typing and deduce their haplotypes.
METHODSBi-allelic sequence-based typing and Luminex DNA PCR-SSOP assay were applied for the initial or repeat HLA typing, respectively. The rare HLA alleles were confirmed with mono-allelic sequence-based typing. Predicted haplotypes of the rare alleles were inferred based on the frequencies of HLA alleles and haplotypes in Han population.
RESULTSThe authenticity of the total 17 rare HLA alleles was proven, and 18 predicted haplotypes associated with the rare alleles were recognized. A*11:12 and DRB1*13:19 were detected twice among unrelated individuals.
CONCLUSIONStudy of rare HLA alleles and predicted haplotype can provide useful information for donor searching and transplantation, and enrich polymorphisms of HLA in this population.
