Application of methylation-specific multiplex ligation-dependent probe amplification for the study of DNA methylation in placenta tissues.
- VernacularTitle:生殖干细胞的研究进展
- Author:
Yuzhu YIN
1
;
Jun ZHANG
;
Qin SHE
;
Qi TIAN
;
Junwei LIN
Author Information
- Publication Type:Journal Article
- MeSH: Actins; genetics; Adult; Carbon-Nitrogen Ligases; genetics; CpG Islands; genetics; DNA Methylation; Endosomal Sorting Complexes Required for Transport; genetics; Feasibility Studies; Female; Humans; Multiplex Polymerase Chain Reaction; methods; Placenta; metabolism; Pregnancy; Reproducibility of Results; Ribosomal Proteins; genetics; Young Adult
- From: Chinese Journal of Medical Genetics 2014;31(5):582-586
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the feasibility of using methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) for the detection of DNA methylation in placenta tissue.
METHODSFor blood cells from 13 non-pregnant women and 9 euploid placenta, the ratios of DNA methylation were evaluated for 4 genes including CGI149, CGI113, HLCS and ACTB with MS-MLPA and bisulfite sequencing, respectively.
RESULTSThe methylation ratio of the ACTB gene was 0-0.1 for the blood cells when the digestion control was completely digested. The cutoff value for the methylation ratio of MS-MLPA has been determined as 0.1. For the 9 placenta samples, results of MS-MLPA and bisulfite sequencing were concordant for all of the four genes.
CONCLUSIONMS-MLPA is an effective alternative to bisulfite sequencing for the assessment of methylation ratios in placental tissues.
