Identification of a novel HLA allele HLA-DRB1*09:01:07.
- Author:
Xiaofeng LI
1
;
Xu ZHANG
;
Kunlian ZHANG
;
Fengqiu LIN
;
Jianping LI
Author Information
- Publication Type:Case Reports
- MeSH: Base Sequence; Blood Donors; HLA-DRB1 Chains; genetics; Histocompatibility Testing; methods; Humans; Molecular Sequence Data; Polymerase Chain Reaction; Polymorphism, Single-Stranded Conformational; Sequence Analysis, DNA
- From: Chinese Journal of Medical Genetics 2014;31(5):663-665
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify and confirm a novel HLA allele in a Chinese Han individual.
METHODSHLA typing was performed by polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSOP) for HLA-A, -B and -DRB1 in a registered donor of China Marrow Donor Program(CMDP). Sequencing-based typing (SBT) was carried out to further confirm the novel allele of HLA-DRB1.
RESULTSThe SSOP result showed HLA-DRB1*09:03,15GEP, but an unusual pattern that could not be defined indicated potential presence of a novel allele. The SBT result showed the novel sequence has 1nt change from its closest allele DRB1*09:01:02 at nt306 where C to T(codon 73 GCC to GCT) resulting in no amino acid change. 73A was not changed.
CONCLUSIONA novel HLA allele, HLA-DRB1*09:01:07, has been identified and named officially by WHO Nomenclature Committee for Factors of the HLA System.
