Analysis of linkage disequilibrium and linkage for 12 short tandem repeat loci on chromosome X.
- Author:
Qiansu YE
1
;
Jianpin TANG
;
Zucong CHEN
;
Fagui LI
;
Xin YU
;
Ping WANG
;
Hanguang LIN
;
Meisen SHI
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Asian Continental Ancestry Group; ethnology; genetics; Child; Child, Preschool; China; ethnology; Chromosomes, Human, X; genetics; Female; Haplotypes; Humans; Linkage Disequilibrium; Male; Microsatellite Repeats; Middle Aged; Pedigree; Young Adult
- From: Chinese Journal of Medical Genetics 2014;31(6):782-785
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze linkage disequilibrium of 12 short tandem repeat loci on chromosome X (X-STR) among an ethnic Han population from Guilin, Guangxi, and to study the genetic linkage and haplotype distributions of such loci in 2 linkage groups.
METHODS12 X-STR loci including DXS8378, DXS10159, DXS10162, DXS10164, DXS981, DXS6789, DXS7424, DXS101, DXS7133, GATA165B12, GATA31E08 and DXS7423 were genotyped using an AGCU X12 STR PCR Amplification kit. A total of 119 pedigrees were analyzed for linkage and linkage disequilibrium.
RESULTSTwo mutations were found at DXS7424, and 1 mutation was found at DXS10164. A total of 93 haplotypes of DXS10159-DXS10162-DXS10164 were constructed for 261 unrelated males and females, in addition with 167 haplotypes of DXS6789-DXS7424-DXS101-DXS7133. The values of recombination fraction between DXS10159 and DXS10162, DXS10162 and DXS10164, DXS6789 and DXS7424, and DXS7424 and DXS101 were 0.0269, 0.0236, 0.0505 and 0.0438, respectively.
CONCLUSIONLinkage disequilibrium of X-STR does not only depend on physical and genetic distances. There was incomplete linkage relationship between loci on DXS10159-DXS1016-DXS10164 and DXS6789-DXS7424-DXS101 linkage groups.
