Nucleotide sequence analysis for a new HLA-B allele HLA-B*13:01:06*.
- Author:
Baiyu XIONG
1
;
Yin TAN
;
Yingfeng HUANG
;
Shaoyu YANG
;
Hongtu LUO
;
Liang SHEN
;
Taichen ZHOU
;
Cangjun XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; Amino Acid Sequence; Base Sequence; Exons; HLA-B Antigens; genetics; Humans; Male; Middle Aged; Molecular Sequence Data; Sequence Analysis, DNA
- From: Chinese Journal of Medical Genetics 2014;31(6):790-792
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo confirm a new allele HLA-B*13:01:06 and analyze its nucleotide sequence.
METHODSGenomic DNA was extracted using a Qiagen DNA extraction kit. Nucleotide sequences of HLA-A, HLA-B, HLA-C and HLA-DRB1 were analyzed by polymerase chain reaction-sequence based typing (PCR-SBT). HLA high-resolution results were assigned, and the nucleotide sequences of HLA-B locus was compared with that of HLA-B*13:01:01.
RESULTSThe nucleotide sequence of the new allele shows a strong similarity to that of HLA-B*13:01:01. One nucleotide in exon 2 has changed from G to A at position 219 (codon 49 GCG>GCA), which however did not result in amino acid change.
CONCLUSIONThe novel allele verified by sequencing has been submitted to GenBank and officially named as HLA-B*13:01:06 by the World Health Organization HLA Nomenclature Committee.
