Polymorphisms of vascular diseases-related genes in Guangzhou Biobank Cohort Study.
- Author:
Bin LIU
1
;
Chao-Qiang JIANG
;
Graham Neil THOMAS
;
Xiang-Qian LAO
;
Jie-Ming LIN
;
Xiao-Jun YUE
;
Wei-Sen ZHANG
;
Tong ZHU
;
Da-Qing LIN
Author Information
- Publication Type:Journal Article
- MeSH: Asian Continental Ancestry Group; genetics; Biological Specimen Banks; statistics & numerical data; Brain Diseases; epidemiology; genetics; Cardiovascular Diseases; epidemiology; genetics; China; epidemiology; Cohort Studies; Genetic Association Studies; Genotype; Humans; Polymorphism, Single Nucleotide
- From: Chinese Journal of Preventive Medicine 2010;44(1):65-69
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the polymorphisms of cerebrovascular and cardiovascular disease genes using Taqman single nucleotide polymorphism (SNP) genotyping kits.
METHODSA total of 2000 subjects were recruited from the Guangzhou Biobank Cohort Study (GBCS), and 15 SNPs were detected using Taqman SNP genotyping kits and an ABI 7900HT real time PCR system. The data were tested for the Hardy-Weinberg equilibrium, and then compared with the data of the Chinese population from the International HapMap Project (HapMap_HCN).
RESULTS(1) All genotype data of the 15 SNPs were consistent with the Hardy-Weinberg rules. (2) The significant differences were observed among two SNPs, rs4220 and rs5368 and the HapMap_HCN (rs4220 28.2% vs 17.8%; chi(2) = 4.891, P = 0.028; rs5368 22.1% vs 32.2%, chi(2) = 5.137, P = 0.024). Comparing other gene bank data, such as AFD-CHN-PANEL, the Allele Frequency Database (ALFRED) and JBIC-allele, it would be most likely that our observations represent differences between the Northern and Southern populations in China.
CONCLUSIONSuch Biobank study provided a useful platform for the study of the role of genetic and environmental determinants on cerebrovascular and cardiovascular disease.
