Effect of cyclin G1 antisense oligodeoxynucleotides (ASON) on the growth of HL-60 cells in nude mice.
- Author:
Jin-song JIA
1
;
Shi-rong XU
;
Cun-rong JIA
;
Jie MA
;
Sen HA
;
Yin-rong YAO
;
Yi WANG
;
Cui-ying SHI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; genetics; Cell Division; genetics; Cyclin G; Cyclin G1; Cyclins; genetics; metabolism; Female; Flow Cytometry; HL-60 Cells; Humans; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplasm Transplantation; Oligonucleotides; genetics; metabolism; Oligonucleotides, Antisense; genetics; metabolism; RNA, Messenger; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Transfection; Xenograft Model Antitumor Assays; methods
- From: Chinese Journal of Hematology 2003;24(12):648-651
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the inhibition effect of cyclin G(1) antisense oligodeoxynucleotides (ASON) on the growth of HL-60 cells in nude mice.
METHODS(1) Nude mice were divided into control group, sense oligodeoxynucleotides (SON) group and ASON group. After (60)Co radiation, with HL-60 cells SON group and ASON group were subcutaneously innoculated; (2) The weight and volume of tumors were continually measured; (3) The morphology of tumor cells was observed by microscope; (4) The protein and mRNA expression levels of cyclin G(1) were determined by flow cytometry (FCM) and reverse transcription polymerase chain reaction (RT-PCR); (5) The cell apoptosis was detected by electron microscopy and FCM.
RESULTS(1) The inhibition rate of tumor in ASON group was 69.4%. In ASON group, the wight and volume of tumor were significantly lower than those in SON group and control group. (2) The HL-60 cells in ASON group showed morphologically smaller nuclei, less mitosis, less heteromorphosis and apoptosis.
CONCLUSIONThe cyclin G(1) ASON can inhibit the growth of HL-60 cells in nude mice and induce apoptosis.
