Isolation and characterization of human placenta derived adherent cells and their hematopoietic growth factor expression.
- Author:
Jin HE
1
;
Yi ZHANG
;
Xiao-xia JIANG
;
Gang LIU
;
Yuan-lin LIU
;
He-lian LI
;
Ning MAO
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Female; Gene Expression; Granulocyte Colony-Stimulating Factor; genetics; Granulocyte-Macrophage Colony-Stimulating Factor; genetics; Hematopoietic Cell Growth Factors; genetics; Humans; Interleukin-6; genetics; Macrophage Colony-Stimulating Factor; genetics; Membrane Proteins; genetics; Placenta; cytology; metabolism; Pregnancy; RNA, Messenger; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Stem Cell Factor; genetics
- From: Chinese Journal of Hematology 2003;24(12):652-654
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo isolate and culture human placenta derived adherent cells (hPDAC) and assay their hematopoietic growth factor expression.
METHODSBy enzyme digestion, hPDAC were isolated from human placenta tissue and cultured, and their biological characteristics were studied. The hematopoietic growth factor (HGF) mRNA expression of hPDAC was assayed by RT-PCR.
RESULTShPDAC was successfully isolated from human placenta tissue, which was further confirmed as mesenchymal stem cell-like cells. HGF including SCF, FL, G-CSF, GM-CSF, M-CSF and IL-6 were expressed in hPDAC.
CONCLUSIONhPDAC could be used as feeder layer for umbilical cord blood CD(34)(+) cells ex vivo expansion.
