Clonal expansion of TCR Vbeta subfamily T cells induced by bcr3-abl2 peptide.
- Author:
Yu-ping ZHANG
1
;
Yang-qiu LI
;
Li-jian YANG
;
Shao-hua CHEN
;
Xue-li ZHANG
;
Zhen WANG
;
Xiu-li WU
;
Geng-xin LUO
Author Information
- Publication Type:Journal Article
- MeSH: Antibodies, Monoclonal; immunology; CD3 Complex; immunology; Fusion Proteins, bcr-abl; pharmacology; Genes, T-Cell Receptor beta; Humans; Interleukin-2; pharmacology; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; immunology; T-Lymphocytes, Cytotoxic; immunology
- From: Chinese Journal of Hematology 2004;25(2):95-99
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the clonal expansion of T cell receptor (TCR) Vbeta subfamily T cells from cord blood induced by bcr3-abl2 peptide in vitro.
METHODST cells from 3 units of cord blood were amplified by anti-CD(3) monoclonal antibody (McAb) and IL-2 with or without synthetic b3a2 peptide. T cell specific cytotoxicity was analyzed by lactate dehydrogenase (LDH) assay, TCR Vbeta subfamilies by using reverse transcriptase-polymerase chain reaction (RT-PCR) and genescan technique.
RESULTSbcr3-abl2 peptide specific cytotoxicity T cells were successfully induced from the 3 units of cord blood by synthetic b3a2 peptide. Compared with that in CD(3) McAb induced cells, distribution pattern of TCR Vbeta repertoire was different in T cells induced with b3a2 peptide. Oligoclonal and oligoclonal tendency TCR Vbeta subfamily T cells could be identified in cord blood T cells induced by b3a2 peptide in 1 or 2 weeks, whereas those induced by anti-CD(3) McAb and IL-2 were mostly polyclonal.
CONCLUSIONThe cytotoxicity T cells with anti-CML specificity could be induced by b3a2 peptide. The specific anti-CML cytotoxicity may be derived from the clonal expansion TCR Vbeta subfamily T cells.
